Fig. 5 Ferroptosis causes specific developmental delay of blood progenitor cells. (A-C) Pseudotemporal analysis of zebrafish blood progenitor cells, erythrocytes and macrophages. (D) Expression of genes related to iron metabolism in blood progenitor cells. (E) Analysis of KEGG enrichment for differentially expressed genes in blood progenitor cells. (F) Subgroup analysis of zebrafish embryos at 12 hpf. (G) Pseudotemporal analysis of sub-cluster groups of blood progenitor cells. (H) EDGs in subcluster 3 related to RNA splicing, mTOR signaling pathway and glutathione metabolism. (I) GO and KEGG enrichment analysis of EDGs in subcluster 3. (J) Immunofluorescence of ferroptosis marker-SO2/3-PRDX3 zebrafish embryos at 12 hpf and 20 hpf.
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