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Fig. 5 CRISPR/Cas9 knockout of hand2, jag1a, and unc5c impaired ENS development and resulted in decreased enteric neuronal number. (a) Representative images of the wild type zebrafish (control), the hand2, jag1a and unc5c crispant zebrafish with number of neurons above the upper (left) and below the lower quantile (right) are shown. The image at the bottom right panel represents the zoomed-in view (indicated by the dotted box) visualizing the Kaede-expressing enteric neurons (green dots) in the intestine. Asterisk (∗) indicates end of the gut tube (anal pore). (b) Significant reduction in the number of enteric neurons in hand2 (n = 80), jag1a (n = 66) and unc5c (n = 72) crispants compared to wild type control fish (n = 63). ∗∗∗ T-test p < 0.001.