FIGURE

Fig. 5

ID

ZDB-FIG-250610-48

Publication

Zhong et al., 2025 - Multi-ancestry genome-wide association meta-analysis identifies novel associations and informs genetic risk prediction for Hirschsprung disease

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Fig. 5

CRISPR/Cas9 knockout of hand2, jag1a, and unc5c impaired ENS development and resulted in decreased enteric neuronal number. (a) Representative images of the wild type zebrafish (control), the hand2, jag1a and unc5c crispant zebrafish with number of neurons above the upper (left) and below the lower quantile (right) are shown. The image at the bottom right panel represents the zoomed-in view (indicated by the dotted box) visualizing the Kaede-expressing enteric neurons (green dots) in the intestine. Asterisk (∗) indicates end of the gut tube (anal pore). (b) Significant reduction in the number of enteric neurons in hand2 (n = 80), jag1a (n = 66) and unc5c (n = 72) crispants compared to wild type control fish (n = 63). ∗∗∗ T-test p < 0.001.

Expression Data

Gene:	Kaede
Fish:	em2Tg em2Tg + CRISPR1-unc5cb em2Tg + CRISPR3-jag1a em2Tg + CRISPR12-hand2
Knockdown Reagents:	CRISPR1-unc5cb CRISPR3-jag1a CRISPR12-hand2
Anatomical Term:	enteric neuron
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	em2Tg + CRISPR1-unc5cb em2Tg + CRISPR3-jag1a em2Tg + CRISPR12-hand2
Knockdown Reagents:	CRISPR1-unc5cb CRISPR3-jag1a CRISPR12-hand2
Observed In:	enteric neuron
Stage:	Day 5

Phenotype Detail

Acknowledgments

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