Figure 1

Figure 1

TMEM184A interacts with Sdc4 proteoglycans in BAOECs. (A) IF staining of TMEM184A NTD (red) merged with Sdc4 CTD (green) with maximum intensity (max, zoom projection) and slice 3 orthogonal (ortho) views of white boxes of slice 3 basal leading edge (LE). Z-position x and y coordinates and crosshairs correspond with white arrowheads in merged ortho images. Scale 20 μm, N = 18 across three independent experiments. (B) IF staining of lentiviral cell lines as described previously in A, with merged max intensity and slice 8 apical cell surface image projections (white boxes). Arrowheads denote x and y coordinates and crosshairs of the slice 8 z-position. Scale 20 μm. N = 6 for both Sdc4-HA and Sdc4-HA-ΔGAG transformations. N = 12 for standard control cells.