Figure 5

Figure 5

TMEM184A colocalizes with recycling Rab-GTPases in the presence of VEGF. BAOECs were treated with VEGF-165 or left untreated as noted, fixed, and permeabilized with 0.5% Triton X-100 to increase the visibility of vesicles. (A) In untreated cells (no tmt), Rab4 (red) colocalizes with TMEM184A NTD (green) in bright yellow puncta (gray box, max intensity projection zoom). Scale 20 μm. Images are representative of 19 Con siRNA images and 17 siTMEM images from four independent experiments. (B) Rab11a (red) colocalizes with TMEM184A NTD (green) in bright yellow and orange puncta (gray box, max intensity projection zoom) in cells without treatment. Scale 20 μm. Images are representative of 11 Control and KD images from two independent experiments. (C) Rab 4 (red), (D) Rab11 (red) and TMEM184A NTD (green) colocalization appears to increase (gray boxes) upon 10 and 20 min VEGF treatment. Scale 20 μm. Images are representative of 13 Con siRNA images and 11 siTMEM images from two independent experiments for C., and 11 images for Control and KD cells across two independent experiments for D.