ZFIN Image: Galuh et al., 2025, Figure 2

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Figure Caption

Figure 2

ZBTB16 silencing in primary CECs affects GR-mediated gene expression. Primary CECs derived from five post-mortem eye donors were transfected with either SMARTpool mix siZBTB16 or siNT for 48 h. Cortisol-induced GR target genes were measured after treatment with 100 nM cortisol (Cort) for 4 h (upper row): (A) FKBP5 (P = 0.04), (B) PER1, (C) ITGA10, (D) ANGPTL4, (E) TSC22D3 (GILZ). The lower row shows repressed genes: (F) CCL2 (P = 0.048), (G) PLAU (P = 0.009), (H) RND1 (P = 0.047), (I) NR3C1. The Ct value of target gene was normalized to LRP10 as a housekeeping gene. The black lines connecting two points show the change between siNT and siZBTB16 conditions within the same donor. ns: not significant.

Acknowledgments

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