IMAGE

Figure 3

ID

ZDB-IMAGE-260316-93

Genes

EGFP

Antibodies

Ab7-sox9

Source

Figures for Xu et al., 2026

Image

Figure Caption

Figure 3

BMP deficiency reduces valvular cell proliferation and Sox9+ cell population. (A) Immunofluorescence detection of Sox9 expression in arterial valvular cells of Tg(7×TCF:EGFP);bmp16 embryos at 96 hpf. Scale bar: 15 µm. (B) Quantification of Sox9⁺ and Sox9⁺; EGFP⁺ valvular cells in wild-type and bmp16 knockout embryos, analyzed using unpaired Student’s t-tests. n = 11–13. (C) Quantification of Sox9⁺; EGFP⁺ valvular cells in vehicle- and LDN-193189-treated embryos, analyzed using unpaired Student’s t-tests. n = 10. (D) EdU labeling in Tg(7×TCF:EGFP) embryos at 72 hpf following vehicle or LDN-193189 treatment. Scale bar: 15 µm. (E) Quantification of EdU⁺; EGFP⁺ arterial valve cells in vehicle- and LDN-193189-treated embryos, analyzed using unpaired Student’s t-tests. n = 10. **, p < 0.01; ****, p < 0.0001.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.