FIGURE

Figure 3

ID

ZDB-FIG-260316-92

Publication

Xu et al., 2026 - Bmp16 Regulates Arterial Valve Morphogenesis Through Modulation of Notch Signaling in Zebrafish

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Figure 3

BMP deficiency reduces valvular cell proliferation and Sox9+ cell population. (A) Immunofluorescence detection of Sox9 expression in arterial valvular cells of Tg(7×TCF:EGFP);bmp16 embryos at 96 hpf. Scale bar: 15 µm. (B) Quantification of Sox9⁺ and Sox9⁺; EGFP⁺ valvular cells in wild-type and bmp16 knockout embryos, analyzed using unpaired Student’s t-tests. n = 11–13. (C) Quantification of Sox9⁺; EGFP⁺ valvular cells in vehicle- and LDN-193189-treated embryos, analyzed using unpaired Student’s t-tests. n = 10. (D) EdU labeling in Tg(7×TCF:EGFP) embryos at 72 hpf following vehicle or LDN-193189 treatment. Scale bar: 15 µm. (E) Quantification of EdU⁺; EGFP⁺ arterial valve cells in vehicle- and LDN-193189-treated embryos, analyzed using unpaired Student’s t-tests. n = 10. **, p < 0.01; ****, p < 0.0001.

Expression Data

Gene:	EGFP
Antibody:	Ab7-sox9
Fish:	ia4Tg/ia4Tg bmp16^{sus206/sus206}; ia4Tg/ia4Tg
Condition:	chemical treatment by environment: LDN-193189
Anatomical Term:	heart valve
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ia4Tg/ia4Tg bmp16^{sus206/sus206}; ia4Tg/ia4Tg
Condition:	chemical treatment by environment: LDN-193189
Observed In:	cell proliferation involved in heart valve development heart valve
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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