PUBLICATION
Functional role for Taz during hindbrain ventricle morphogenesis
- Authors
- Dicipulo, R., Selland, L.G., Carpenter, R.G., Waskiewicz, A.J.
- ID
- ZDB-PUB-250315-3
- Date
- 2025
- Source
- PLoS One 20: e0313262e0313262 (Journal)
- Registered Authors
- Waskiewicz, Andrew
- Keywords
- none
- MeSH Terms
-
- Transcriptional Coactivator with PDZ-Binding Motif Proteins
- Serine-Threonine Kinase 3
- Cell Polarity
- Gene Expression Regulation, Developmental
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Signal Transduction
- Zebrafish*/embryology
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Animals
- Cerebral Ventricles/embryology
- Cerebral Ventricles/metabolism
- Morphogenesis*
- Actins/metabolism
- Rhombencephalon*/embryology
- Rhombencephalon*/metabolism
- PubMed
- 40080483 Full text @ PLoS One
Citation
Dicipulo, R., Selland, L.G., Carpenter, R.G., Waskiewicz, A.J. (2025) Functional role for Taz during hindbrain ventricle morphogenesis. PLoS One. 20:e0313262e0313262.
Abstract
The brain ventricle system, composed of the ventricular cavities and the cerebral spinal fluid within, performs critical functions including circulation of nutrients, removal of wastes, and cushioning of neural tissues. Development of the hindbrain ventricle requires a series of factors that coordinate its initial formation and subsequent inflation. Previous work has demonstrated that the transcriptional co-activator Taz (also known as WW domain-containing transcription regulator protein 1, Wwtr1), a component of Hippo signalling, is active at hindbrain rhombomere boundaries where it is regulated by mechanotransduction and promotes proliferation. Here, we demonstrate that Taz is also a critical regulator of hindbrain ventricle development. Zebrafish embryos that lack Taz protein fail to undergo initial midline separation of the hindbrain ventricle. Furthermore, the ventricle phenotype is a result of disorganized cytoskeletal F-actin and apicobasal polarity components. In addition, we have demonstrated that the hindbrain rhombomere boundaries are a location of active Wnt-Hippo crosstalk. Through our work, we propose a model where Taz protein is stabilized at rhombomere boundaries and promotes proper cell polarity necessary for formation of the brain ventricle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping