PUBLICATION
Rhodopsin Induces Myopia via Lipid Peroxidation in Zebrafish Reared in a Dark Environment
- Authors
- Liu, S., Fang, W., Pei, Y., Chen, T., Li, B., Liang, Y., Lu, X., Li, J.
- ID
- ZDB-PUB-250511-1
- Date
- 2025
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39: e70612e70612 (Journal)
- Registered Authors
- Keywords
- 4?HNE, lipid peroxidation, myopia, rhodopsin, zebrafish
- MeSH Terms
-
- Darkness
- Animals
- Myopia*/etiology
- Myopia*/metabolism
- Myopia*/pathology
- Zebrafish/metabolism
- Apoptosis
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Retinal Pigment Epithelium/metabolism
- Rhodopsin*/genetics
- Rhodopsin*/metabolism
- Lipid Peroxidation*
- PubMed
- 40347036 Full text @ FASEB J.
Citation
Liu, S., Fang, W., Pei, Y., Chen, T., Li, B., Liang, Y., Lu, X., Li, J. (2025) Rhodopsin Induces Myopia via Lipid Peroxidation in Zebrafish Reared in a Dark Environment. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 39:e70612e70612.
Abstract
Outdoor time and light intensity are important emerging factors affecting myopia; however, the underlying mechanisms remain unknown. This study aimed to clarify whether a dark environment induces myopia in zebrafish and investigate the role of rhodopsin in this process. To this end, zebrafish were reared in a dark environment, and myopia-related parameters were measured. RNA sequencing and histological analysis were performed. We found a myopic shift with a relative refractive error (RRE) change and an increased eye-to-body length ratio in zebrafish reared in the dark compared to that in their light-reared siblings. RNA sequencing indicated that the phototransduction pathway was affected. Hematoxylin-eosin staining and immunofluorescence analysis confirmed the elongation of the rod's outer segment and increased rhodopsin expression. Overexpressed rhodopsin determined a myopic RRE; ocular enlargement resembled myopia and elevated 4-hydroxynonenal (4-HNE), which was also present in the retinal pigment epithelium of the dark-reared zebrafish. Incubation of ARPE-19 cells with a low 4-HNE concentration increased cell proliferation capacity, while incubation of ARPE-19 cells with its high concentration induced apoptosis. The increased expression of rhodopsin in a dark-rearing environment affected refractive development in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping