PUBLICATION
Development of Zebrafish model for Iron Induced Neuroinflammation
- Authors
- Bagwe Parab, S., Kaur, G.
- ID
- ZDB-PUB-250909-9
- Date
- 2025
- Source
- Fish physiology and biochemistry 51: 160160 (Journal)
- Registered Authors
- Keywords
- Acetylcholinesterase, Brain iron overload, Neuroinflammation, Novel tank test, Spatial memory, Wild type zebrafish, Y maze test
- MeSH Terms
-
- Ferrous Compounds*/toxicity
- Disease Models, Animal*
- Interleukin-1beta/metabolism
- Zebrafish*
- Animals
- Behavior, Animal/drug effects
- Brain/drug effects
- Brain/metabolism
- Neuroinflammatory Diseases*/chemically induced
- Neuroinflammatory Diseases*/metabolism
- Iron*/metabolism
- Iron*/toxicity
- Acetylcholinesterase/metabolism
- Oxidative Stress/drug effects
- PubMed
- 40920220 Full text @ Fish Physiol. Biochem.
Citation
Bagwe Parab, S., Kaur, G. (2025) Development of Zebrafish model for Iron Induced Neuroinflammation. Fish physiology and biochemistry. 51:160160.
Abstract
Zebrafish models have been used to research Alzheimer's disease and other neurodegenerative disorders because of their similarities to the human genetic composition and behavior. Researchers have detected iron accumulation in the post-mortem brain sections of neurodegenerative disorder patients. Therefore, the development an animal model to simulate these clinical pathological findings is important. Iron is an important metal for maintaining homeostasis in the brain, depletion and accumulation of iron hamper neuronal development. Given the importance of iron overload in cognition impairment, this research aimed to develop an iron-induced zebrafish model of cognitive impairment. Zebrafish were subjected to ferrous sulfate (1.5 mg/L, 3 mg/L, and 6 mg/L) for 28 days. The behavioral parameters (Y-maze, novel tank test), oxidative stress parameters (MDA, GSH, and catalase), acetylcholinesterase (AChE) levels, iron levels, and interleukin-1β (IL-1β) levels in brain homogenate were assessed. The behavioral and locomotor responses, specifically in the zebrafish treated with iron for 28 days, suggest an increase in the loss of spatial memory and anxiety. Reactive oxygen species in the brain significantly increased (p < 0.001) with the increase in concentrations of iron. Brain tissue iron content and IL-1β significantly increased (p < 0.001) in the brain homogenate of the zebrafish. This model will aid in the screening of therapeutic compounds to accelerate drug discovery in the field of neurodegenerative diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping