Publication	Data		Fish	Anatomy
Yin et al., 2020	Fig. 4		WT + MO3-nkx2.5	anterior lateral plate mesoderm atf3 expression decreased amount, abnormal atrium dilated, abnormal
Gao et al., 2019	Fig. 2		AB + MO2-nkx2.5	whole organism gdf3 expression decreased amount, abnormal
Colombo et al., 2017	Fig. 2		nkx2.5vu179/vu179	heart tube mef2cb expression decreased amount, abnormal
	Fig. 3		nkx2.5vu179/vu179; fb7Tg	cardiac muscle myoblast cell population proliferation disrupted, abnormal
	Fig. 4		nkx2.5vu179/vu179	atrium isl1a expression increased distribution, abnormal atrium isl1a expression mislocalised, abnormal atrium isl1a expression spatial pattern, abnormal
	Fig. 5		nkx2.5vu179/vu179 ,   nkx2.5vu179/vu179; fb7Tg	atrium isl1a expression increased distribution, abnormal atrium isl1a expression mislocalised, abnormal atrium isl1a expression spatial pattern, abnormal presumptive atrium heart tube isl1a expression increased distribution, abnormal presumptive atrium heart tube isl1a expression spatial pattern, abnormal
	Fig. 7		nkx2.5vu179/vu179	myocardium bmp4 expression increased amount, abnormal myocardium bmp4 expression increased distribution, abnormal myocardium bmp4 expression spatial pattern, abnormal myocardium hcn4 expression increased amount, abnormal myocardium hcn4 expression increased distribution, abnormal myocardium hcn4 expression spatial pattern, abnormal myocardium tbx2b expression increased amount, abnormal myocardium tbx2b expression increased distribution, abnormal myocardium tbx2b expression spatial pattern, abnormal
	Fig. 8		nkx2.5vu179/vu179	myocardium bmp4 expression increased amount, abnormal myocardium bmp4 expression increased distribution, abnormal myocardium bmp4 expression spatial pattern, abnormal
	Fig. S2		nkx2.5vu179/vu179	atrium shox2 expression increased distribution, abnormal atrium shox2 expression mislocalised, abnormal atrium shox2 expression spatial pattern, abnormal cardiac ventricle shox2 expression increased amount, abnormal cardiac ventricle shox2 expression increased distribution, abnormal cardiac ventricle shox2 expression spatial pattern, abnormal myocardium shox2 expression increased amount, abnormal myocardium shox2 expression increased distribution, abnormal
Harrington et al., 2017	Fig. 2		nkx2.5vu179/vu179	heart contraction increased rate, abnormal
	Fig. 3		nkx2.5vu179/vu179	heart decreased functionality, abnormal
	Fig. text only		nkx2.5vu179/vu179	heart edematous, abnormal whole organism dead, abnormal whole organism decreased life span, abnormal
Iklé et al., 2017	Fig. 5		co10Tg + MO2-nkx2.5 ,   WT + MO2-nkx2.5 ,   nkx2.5vu179/vu179	basihyal cartilage decreased size, abnormal ceratobranchial cartilage hypoplastic, abnormal cranial cartilage hypoplastic, abnormal hyosymplectic cartilage hypoplastic, abnormal Meckel's cartilage curved ventral, abnormal palatoquadrate cartilage malformed, abnormal pharyngeal arch ventral region mCherry expression decreased distribution, abnormal
	Fig. 6		WT + MO2-nkx2.5	pharyngeal arch 1 dlx3b expression absent, abnormal pharyngeal arch 1 dlx6a expression decreased distribution, abnormal pharyngeal arch 1 hand2 expression absent, abnormal pharyngeal arch 2 dlx3b expression absent, abnormal pharyngeal arch 2 dlx6a expression decreased distribution, abnormal pharyngeal arch 2 hand2 expression absent, abnormal pharyngeal arch 3 hand2 expression decreased distribution, abnormal pharyngeal arch 4 hand2 expression decreased distribution, abnormal pharyngeal arch dlx2a expression increased distribution, abnormal pharyngeal arch ece1 expression decreased distribution, abnormal pharyngeal arch edn1 expression decreased distribution, abnormal whole organism dlx3b expression decreased amount, abnormal whole organism dlx5a expression decreased amount, abnormal whole organism dlx6a expression decreased amount, abnormal whole organism edn1 expression decreased amount, abnormal whole organism nkx2.7 expression decreased amount, abnormal
	Fig. 7		WT + MO2-nkx2.5	cranial cartilage hypoplastic, abnormal Meckel's cartilage curved ventral, abnormal pharyngeal arch hand2 expression decreased distribution, abnormal
	text only		WT + MO2-nkx2.5 + MO4-tp53	basihyal cartilage decreased size, abnormal ceratobranchial cartilage hypoplastic, abnormal cranial cartilage hypoplastic, abnormal hyosymplectic cartilage hypoplastic, abnormal Meckel's cartilage curved ventral, abnormal palatoquadrate cartilage malformed, abnormal
George et al., 2015	Fig. 2		nkx2.5vu179/vu179	atrium increased size, abnormal atrium morphology, abnormal cardiac ventricle decreased size, abnormal cardiac ventricle morphology, abnormal heart looping decreased process quality, abnormal heart morphology, abnormal pericardium edematous, abnormal
	Fig. 3		nkx2.5vu179/vu179	heart looping decreased process quality, abnormal heart morphology, abnormal
	Fig. 7		nkx2.5vu179/vu179	whole organism decreased life span, abnormal
Nagelberg et al., 2015	Fig. 5		nkx2.5vu179/vu179; fb7Tg; s896Tg	aortic arch 3 ZsYellow expression decreased amount, abnormal aortic arch 5 blood vessel lumenization decreased occurrence, abnormal aortic arch 6 blood vessel lumenization decreased occurrence, abnormal
Paffett-Lugassy et al., 2013	Fig. 3		la116Tg; sd2Tg + MO3-nkx2.5	pharyngeal vasculature malformed, abnormal pharyngeal vasculature pharyngeal arch artery morphogenesis decreased process quality, abnormal pharyngeal vasculature vasculogenesis decreased process quality, abnormal
	Fig. 4		WT + MO3-nkx2.5 ,   fb7Tg; y7Tg + MO3-nkx2.5	pharyngeal vasculature blood vessel endothelial cell differentiation decreased occurrence, abnormal pharyngeal vasculature has fewer parts of type blood vessel endothelial cell, abnormal
	Fig. 5		WT + MO3-nkx2.5	pharyngeal vasculature angioblast cell differentiation decreased process quality, abnormal
Targoff et al., 2013	Fig. 1		nkx2.5vu179/vu179	atrial myocardium bulbous, abnormal atrial myocardium dilated, abnormal atrial myocardium distended, abnormal atrial myocardium increased width, abnormal heart morphology, abnormal pericardial cavity edematous, abnormal ventricular myocardium decreased size, abnormal ventricular myocardium decreased width, abnormal
	Fig. 2		nkx2.5vu179/vu179; f2Tg	atrial myocardium cardiac muscle cell increased amount, abnormal ventricular myocardium cardiac muscle cell decreased amount, abnormal
	Fig. 3		nkx2.5vu179/vu179	atrial myocardium increased size, abnormal ventricular myocardium decreased size, abnormal
	Fig. 4		nkx2.5vu179/vu179	atrial myocardium increased size, abnormal ventricular myocardium decreased size, abnormal
	Fig. 5		nkx2.5vu179/vu179	atrial myocardium increased width, abnormal ventricular myocardium shortened, abnormal
	Fig. 6		nkx2.5vu179/vu179	atrial myocardium dilated, abnormal atrial myocardium increased size, abnormal ventricular myocardium decreased size, abnormal ventricular myocardium shortened, abnormal
Tu et al., 2009	text only		twu34Tg + MO2-nkx2.5	pericardium edematous, abnormal
Sultana et al., 2008	Fig. S8		WT + MO1-nkx2.5	heart development disrupted, abnormal myocardium myofibril disorganized, abnormal pericardium edematous, abnormal
	text only		WT + MO1-nkx2.5	atrium dilated, abnormal blood circulation disrupted, abnormal cardiac ventricle dilated, abnormal heart contraction decreased rate, abnormal heart contraction irregular rhythm, abnormal
Targoff et al., 2008	Fig. 1		WT + MO2-nkx2.5	atrium shape, abnormal cardiac ventricle decreased size, abnormal
	text only		WT + MO3-nkx2.5	atrium shape, abnormal cardiac ventricle decreased size, abnormal