FIGURE

Fig. 1

ID

ZDB-FIG-170208-28

Publication

Shamay-Ramot et al., 2015 - Fmrp Interacts with Adar and Regulates RNA Editing, Synaptic Density and Locomotor Activity in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Increased expression of Fmrp-target genes in fmr1-/- zebrafish.

A. The full mRNA sequence of the fmr1 gene, including the CDS (black bars) and UTRs (white bars). A single C-to-T mutation at position 113 results in a premature stop codon and truncated protein (gray bars). B-D. Whole-mount ISH assays show the spatial expression of mtor, sash1, and tln1 in 6 dpf WT larvae. Fb, forebrain; Mb, midbrain; Hb, hindbrain. E. Relative mRNA expression of mtor, sash1, and tln1 in 6 dpf fmr1-/- (grey bars) and WT larvae (white bars). Values are represented as means ± SEM (*p<0.05, two-way t-test assuming unequal variances). F. Western blots show an approximate five-fold increase in the expression of mTor protein levels in fmr1-/- zebrafish brain tissue.

Expression Data

Genes:	adar adarb1a adarb1b adarb2 mtor sash1a tln1
Antibodies:	Ab1-adarb1 Ab2-mtor
Fish:	WT fmr1^{hu2787/hu2787}
Anatomical Terms:	forebrain hindbrain midbrain whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fmr1^{hu2787/hu2787}
Observed In:	whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.