FIGURE

Fig. 2

ID

ZDB-FIG-171117-2

Publication

Lee et al., 2016 - Myc and Fgf Are Required for Zebrafish Neuromast Hair Cell Regeneration

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Fig. 2

Myc inhibition suppresses HC regeneration in zebrafish neuromasts.

(A) HCS1-labeled HC number was significantly reduced after treatment by c-MYC inhibitor 10058F4 that was dose-dependent. ***p<0.001. n = 20 larvae for each group. (B) A significant reduction in HC number was observed after the treatment by Int-H1-S6A, F8A, a peptide inhibitor of c-MYC, which was dose-dependent. ***p<0.001. n = 15 larvae for each group. Three independent experiments were performed for each comparison study with similar results. (C-H) Inhibitor Int-H1-S6A, F8A blocked the expression of c-myc target gene odc1. In situ hybridization showed up-regulation of odc1 expression in the neuromast 3 or 6 hrs after neomycin treatment (D, G); whereas Int-H1-S6A, F8A markedly suppressed odc1 expression (E, H). Scale bars: 10 μm.

Expression Data

Gene:	odc1
Fish:	AB
Condition:	chemical ablation: neuromast hair cell, chemical treatment by environment: neomycin
Anatomical Terms:	neuromast neuromast support cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	chemical treatment: thiazolidinone, chemical treatment by environment: neomycin chemical ablation: neuromast hair cell, chemical treatment by environment: neomycin
Observed In:	neuromast hair cell neuromast support cell
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

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