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Relative gene expression in translation-blocking appbMO and appb−/−. A, Relative expression level of appa, appb, aplp2, and aplp1 (N = 10) in morphants compared with wild type (WT; N = 10) at 24 hpf. B, Western blot analysis of Appb and GAPDH levels in wild type and translation-blocking appbMO at 3 dpf. C, Quantification of Western blot data. D, Relative expression of appa, appb, aplp1, and aplp2 at 24 hpf in genetic appb−/− (N = 14) compared with WT (N = 14). E, Relative expression of appa, appb, aplp1, and aplp2 in adult brain of genetic appb−/− mutants (N = 13) and wild-type siblings (N = 12). Wild-type expression levels were set at 1. Data shown as mean + SD. A–E, n = 3 biologically independent samples. Student's two-tailed t test was used to calculate p values. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.001. Additional data relating to these analyses are provided in Extended Data Figure 2-1.
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