FIGURE

Figure 7.

ID

ZDB-FIG-240612-7

Publication

Rahmati et al., 2024 - Fine-tuning amyloid precursor protein expression through nonsense mediated mRNA decay

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Figure 7.

Expression of app family genes in wild-type and appb^−/− embryos before and after knocking down NMD factors. A–C, Relative mRNA expression levels of appb, appa, aplp1, and aplp2 in uninjected wild-type embryos (N = 12–15), uninjected appb^−/− (N = 13–15), wild type injected with upf1MO (N = 12), upf2MO (N = 14), upf3a/upf3bMO (N = 13), and appb^−/− injected with upf1MO (N = 11), upf2MO (N = 12), and upf3a/upf3bMO (N = 15). n = 3 biologically independent samples. Data shown as mean + SD. Student's two-tailed t test were used to calculate p values. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.0001. ns, nonsignificant.

Expression Data

Genes:	aplp1 aplp2 appa appb
Fish:	AB appb^{zf3355/zf3355} (AB) AB + MO1-upf1 AB + MO2-upf2 appb^{zf3355/zf3355} + MO1-upf1 (AB) appb^{zf3355/zf3355} + MO2-upf2 (AB) AB + MO1-upf3a + MO3-upf3b appb^{zf3355/zf3355} + MO1-upf3a + MO3-upf3b (AB)
Knockdown Reagents:	MO1-upf1 MO1-upf3a MO2-upf2 MO3-upf3b
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	appb^{zf3355/zf3355} (AB) AB + MO1-upf1 AB + MO2-upf2 appb^{zf3355/zf3355} + MO1-upf1 (AB) appb^{zf3355/zf3355} + MO2-upf2 (AB) AB + MO1-upf3a + MO3-upf3b appb^{zf3355/zf3355} + MO1-upf3a + MO3-upf3b (AB)
Knockdown Reagents:	MO1-upf1 MO1-upf3a MO2-upf2 MO3-upf3b
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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