FIGURE

Fig. 3

ID

ZDB-FIG-240703-3

Publication

Webb et al., 2021 - EHD2 modulates Dll4 endocytosis during blood vessel development

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Fig. 3

EHD2 and caveolae localize to adherens junctions in sprouts. (A) Schematic of 3-dimensional sprout growth in fibrin-bead assay (FBA) using Human umbilical vein endothelial cells. (B) Representative sprout stained for VE-cadherin (VE-Cad) expressing pHluorin-Dll4 (pH-Dll4). Yellow line marks line scan area. (C) Line scan of pH-Dll4 and VE-Cad of image in panel B. (D) Representative sprout expressing pH-Dll4 and red fluorescence protein (RFP)-EHD2. Yellow line marks line scan area. (E) Line scan of pH-Dll4 and RFP-EHD2 of image in panel D. (F) Representative sprout stained for VE-Cad expressing RFP-EHD2. Yellow line marks line scan area. (G) Line scan of pH-Dll4 and VE-Cad of image in panel F. (H) Representative image of retinal blood vessels in P6 mouse stained for isolectin, Dll4, and caveolin-1 (Cav1). Boxes are magnified images on right. Yellow line marks line scan area. (I) Line scan of Cav1 and Dll4 in panel H. Yellow arrowheads show areas of Dll4 puncta. All experiments were done at minimum in triplicate. All images are a single confocal slice

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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