Fxn-deficient zebrafish embryos display renal clearance defects and some proximal reabsorption defects. (A)fxn morphants failed to excrete the FITC-Dextran at the same rate as their wild-type siblings. Images of the same wild-type and fxn-deficient animals 6 hours and 48 h after injection with the FITC-dextran. FITC-Dextran (40 kDa) was injected around 36 h post-fertilization. (B) An unpaired t-test of the percent fluorescent change of each animal. Percent fluorescent change was calculated using the 6 h post injection (hpi) and 48 hpi fluorescent intensity of the same animal. (C) Morphants exhibit decreased endocytosis activity in the proximal convoluted tubule (PCT). Among the morphants, there were three primary phenotypes. Morphant animals either had endocytosis activity in two PCTs, one PCT, or neither. These findings suggest that dysfunction within the PCT segments is one contributor to the fluid imbalance observed in fxn-deficient animals. (D) The proportions of proximal convoluted tubule phenotypes in the wild-type and fxn morphant groups. Scale bars are 200 um. ***p < 0.0005.
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