Tail/pronephros tubule ratio comparisons indicate that the embryonic kidney that develops within fxn-deficient zebrafish embryos was decreased in length proportionally to the decreased in tail length. (A) WISH analysis of pronephros tubule formation as marked by cdh17 revealed that this structure was shorter in fxn morphants than it is in their wild-type siblings (scale bars = 50 um). Each boxed lateral region in the embryos corresponds to the inset, which shows an enlarged lateral view of that corresponding area. (B) Unpaired t-tests of tail length and pronephros length as marked by cdh17 revealed that the length of both structures was significantly shorter in the morphants than in their wild-type siblings. An unpaired t-test of the ratios of tail and pronephros length also showed a statistically significant difference. *p < 0.05, p ****p < 0.0001. (C)fxn deficiency leads to formation of a pronephros which is decreased in size in porportion to the reduction in embryo size, however the distal segments are disporportionally reduced suggesting these lineages are uniquely affected by the loss of Fxn activity.
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