FIGURE

Fig. 1

ID

ZDB-FIG-250305-19

Publication

Ma et al., 2025 - Establishment and application of a zebrafish model of Werner syndrome identifies sapanisertib as a potential antiaging drug

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Fig. 1

The met mutant with wrn gene mutation exhibits cellular senescence. (A) Bright-field images showing the morphological appearance of WT and met mutants at 8 dpf. (B) Representative image of SA-β-Gal staining and quantification of the intensity of SA-β-Gal staining in the intestine at 8 dpf (n = 12 for each group). Arrows indicate the intestine region. (C) Genetic map of the candidate region on chromosome 10. Numbers below SSLP indicate recombination events. (D) Diagram showing the genomic structure of wrn between WT and met. (E) Schematic representation of the change in function domains of Wrn between WT and met. (F) The genomic sequencing results displaying the mutation site in wrn. Note that the T to A transition (red dashed box) leading a premature stop codon within the coding region in the met mutant. Data are presented as mean ± SEM. Significance was determined by the unpaired t test. ****P < 0.0001. BF, bright-field; dpf, day postfertilization; WT, wild type; SSLP, simple sequence length polymorphism.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wrn^cq216/cq216 (AB)
Observed In:	intestine cellular senescence
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA