Figure 3

Complementary cytosine editing mediated by zTadA‐BEmv and zTadA‐BEmax. A) Schematic of the mRNA construct for zTadA‐BEmv. B) Comparison of the targeting window between zTadA‐BEmv (bottom) and zTadA‐BE4max (top) targeting six different loci with NGG PAM. C) Comparison of targeting window and editing efficiency between zTadA‐BEmax targeting twenty‐two NGG PAM sites across fifteen genes and zTadA‐BEmv targeting eleven NGG PAM sites across seven genes. The editing window of each tool is outlined by two dashed lines, and the average editing efficiency value for each cytosine locus is shown. D) Schematic diagram of the hps1 Q140^* target locus. The targeted sequence is shown with the PAM highlighted in red. The targeted cytosine nucleotide and expected changes are highlighted in green and red, respectively, while the targeted amino acid and expected changes in amino acid are indicated with bold text. E) Sequencing results of F0 embryo after editing with zTadA‐BE4max. The red arrowhead indicates the expected nucleotide substitutions. F) Lateral view of 3 dpf F1 homozygous embryos with the hps1 (Q140^*) mutation (bottom), exhibiting pigmentation defects compared to the wild‐type (top). Scale bar: 500 µm.