Fig. 3

Locomotor activity of atad3-null larvae at 5 dpf and 6 dpf is reduced. Mutants in blue, siblings in grey. (A) Activity over time at 5 dpf: average distance moved per minute over 6 h (light-dark-light cycle, 2 h each). Upper panel indicates light/dark conditions. (B) Mutants demonstrate reduced activity, as measured by average distance moved over 6 h (p < 0.001). (C) Response to tap in mutants compared to siblings, average of 5 and 6 dpf. Average velocity of mutant larvae immediately after tap (0 s on the X axis) is lower than siblings. (D) Average distance moved in the first 0.5 s following tap is 1.4-times lower in mutant larvae compared to siblings (p = 0.012). (E) Average velocity before and after light-off. (F) Initial light-off response: average distance in the first 0.5 s following light-off is reduced in mutants (p < 0.001). (G) Average distance moved over 5 min after light-off switch shows no difference between mutant and sibling activity (p = 0.67). Asterisks represent levels of significance (*p < 0.05, **p < 0.01, ***p < 0.001)