FIGURE

Figure 1

ID

ZDB-FIG-260101-71

Publication

Che et al., 2025 - The Mutation of myomiR miR499 Impacts the Intermuscular Bones in Zebrafish

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Figure 1

Generation and characterization of miR499 knockout. (A) Schematic illustration of miR499 mutation induction by the CRISPR/Cas9 system with two sgRNAs, and sequencing results of WT and miR499 mutant (F2) with 4—bp deletion (named miR499^−/−). Underlined and italicized text indicates the sgRNA target sequence. Green font denotes the pre-miRNA-499 sequence. (B) Semi-quantitative RT-PCR analysis of dre—miR499—5P and dre—miR499—3P expression in WT and miR499^−/− fish, with U6 as a loading control. *** p < 0.001. ns: no significance; (C) Representative images of WT and miR499^−/− fish at 30 days post-fertilization. Scale bars = 50 mm.

Expression Data

Gene:	mir499a
Fish:	AB mir499a^sou23/sou23 (AB)
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mir499a^sou23/sou23 (AB)
Observed In:	whole organism
Stage Range:	Day 6 to Days 30-44

Phenotype Detail

Acknowledgments

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