cripDM embryos recover from embryonic HSPC defects. (A-H) In situ hybridization for cmyb in wild-type (A-D) and cripDM (E-H) embryos. Wild-type (n=45) (A) and cripDM (n=41) (E) embryos at 32 hpf, wild-type (n=40) (B) and cripDM (n=42) (F) embryos at 52 hpf, wild-type (n=39) (C) and cripDM (n=41) (G) embryos at 72 hpf, and wild-type (n=21) (D) and cripDM (n=21) (F) embryos at 4 dpf. Lateral views, anterior to the left. Scale bars: 100 µm. (I,J) Quantification of cmyb signal from A, E, B and F using pixel intensity analysis shows a reduced signal in cripDM embryos employing unpaired nonparametric Mann–Whitney U-tests (**P=0.0049 in I and ****P<0.0001 in J). (K) Quantification of cmyb signal from C and G using pixel intensity analysis shows a reduced signal in cripDM embryos employing an unpaired two-tailed nonparametric t-test (****P<0.001). (L) A similar comparison from D and H illustrates no statistically significant difference (ns) between wild-type and cripDM embryos. Mean and standard error of each dataset are shown. (M) Representative flow cytometry analysis of whole kidney marrow (n=15 for wild-type and n=15 for cripDM fish). Forward and side scatter parameters were used to define the major blood cell populations (erythroid, myeloid, lymphoid and precursors). (N) Quantification of the frequency of blood cell populations isolated from whole kidney marrow. Mean of each dataset is shown.
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