FIGURE

Fig. 4

ID

ZDB-FIG-260406-31

Publication

Jacquinet et al., 2026 - Variants in NR6A1 as a cause for congenital renal, vertebral and uterine anomalies

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Fig. 4

Abnormal expression of posterior Hox in nr6a1a−/− and nr6a1a−/−nr6a1b−/− mutants. a Whole-mount is situ hybridization with hoxa9b at 31hpf in embryos from an incross between nr6a1a+/-nr6a1b+/- and nr6a1a+/-nr6a1b-/- fish. A longer anterior axial staining (neural tube) was observed in ¼ of the stained embryo (18/68). Genotyping was performed for 20 embryos (short phenotype: n = 12; long phenotype: n = 8). b Whole-mount in situ hybridization with hoxa11b, hoxd11a, hoxa13b at 18hpf in embryos from an incross between nr6a1a+/-nr6a1b-/- showing an extended anterior staining in nr6a1a-/- mutants. Genotyping was performed for 8 embryos for hoxa11b (staining as expected: n = 4; extended anterior staining: n = 4), 8 embryos for hoxd11a (staining as expected: n = 4; extended anterior staining: n = 4), 9 embryos for hoxa13b (staining as expected: n = 4; extended anterior staining: n = 5). c Whole-mount is situ hybridization with hoxb13a at 24hpf in embryos from an incross between nr6a1a+/-nr6a1b+/- double heterozygotes. Classification of the embryos following the extend of the staining in the posterior mesendoderm (from absent to severely extended). Genotyping was performed for 19 embryos. Created in BioRender. Humaine, G. (2025)

Expression Data

Genes:	hoxa9b hoxa11b hoxa13b hoxb13a hoxd11a
Fish:	WT nr6a1a^{ulg083/ulg083} nr6a1b^{ulg085/ulg085} nr6a1b^ulg085/+ nr6a1a^ulg083/+; nr6a1b^{ulg085/ulg085} nr6a1a^{ulg083/ulg083}; nr6a1b^ulg085/+ nr6a1a^{ulg083/ulg083}; nr6a1b^{ulg085/ulg085}
Anatomical Terms:	neural tube neural tube posterior region spinal cord
Stage Range:	14-19 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nr6a1a^{ulg083/ulg083} nr6a1a^{ulg083/ulg083}; nr6a1b^ulg085/+ nr6a1a^{ulg083/ulg083}; nr6a1b^{ulg085/ulg085}
Observed In:	neural tube posterior region spinal cord
Stage Range:	14-19 somites to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Genet.