IMAGE

Figure 5

ID

ZDB-IMAGE-200130-19

Genes

Publication

Chen et al., 2019 - Lysophosphatidic acid receptor LPA₃ prevents oxidative stress and cellular senescence in Hutchinson-Gilford progeria syndrome

All Figures

Figures for Chen et al., 2019

Image

Figure Caption

Figure 5

LPA₃ deficiency in zebrafish leads to premature aging phenotypes. (a) Kaplan–Meier survival analysis demonstrated a shorter lifespan in Lpa₃^−/− zebrafish than in corresponding WT siblings (p value = .011, N > 8). (b) Histological analysis shows hematopoietic stem cells loss in kidney marrow of 6‐month‐old Lpa₃^−/− zebrafish. (c) Sperm loss in the testis. (d) Bile duct hyperplasia. (e) Islet hyperplasia in the pancreas. (f) By senescence‐associated β‐gal staining assay, Lpa₃^−/− larvae show a positive β‐gal signal. (g) Representative images of zebrafish swimming tracks obtained and analyzed by locomotor. Locomotor results show that Lpa₃^−/− larvae had lower mobility than WT upon drastic light onset and offset. (h) CM‐DCFDA staining showed increased ROS levels in 5 dpf Lpa₃^−/− larvae. (i) Real‐time qPCR showed enhanced p16 and p21, whereas reduced Gpx1a, SOD2, and NQO1 of 5 dpf Lpa₃^−/− larvae. β‐Actin was used as internal control. ANOVA and Student's t test; *p < .05, ***p < .001

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Aging Cell