Figure 6

Figure 6

Effect on the phases of cell cycle in the SH-SY5Y cells treated with OA. (A)—The cell cycle distribution of PI-labelled cells was analyzed by flow cytometry in the SH-SY5Y control cells and cells treated with OA (715 µg/mL) for 48 h. (B)—The histogram shows the percentage of cells that are in different phases of the cell cycle. The results are expressed as the mean ± SD of three independent experiments. The p values below 0.05 (* p < 0.05, ** p < 0.0001) are considered statistically significant. The treated SH-SY5Y cells were arrested in the G1 phase and the percentage of cells in this phase increased from 16.87 ± 0.68% in the untreated cells to 23.57 ± 1.36% in the cells treated with oleanolic acid. Incubation with OA also caused an increase in the proportion of cells in the S phase (36.18 ± 1.50% vs. 40.85 ± 0.73%, p = 0.023) and a significant decrease in the G2/M phase (27.82 ± 0.86% vs. 17.02 ± 1.75%, p < 0.0001) of the cell cycle in the treated SH-SY5Y cells in comparison with the control.