|
Fig. 8 Hsf1 is required for the muscle-specific upregulation of hsp chaperone and cochaperone gene expression in smyd1b mutant embryos. Expression of three hsp70 genes (hsp70.1/2/l, hspa8b, and hsc70), hsp40, hsp90?1 and unc45b mRNAs was analyzed in the WT control (A?F), hsf1?/? mutants (G?L), smyd1b?/? mutants (M?R) and smyd1b?/?; hsf1?/? double-mutant (S?X) embryos. No significant difference is visible between the WT (A-F) and hsf1?/? mutant (G?L) embryos. In contrast, loss of smyd1b dramatically upregulated the hsp chaperone and cochaperone gene expression in muscle cells of zebrafish embryos at 24 hpf (M?R). However, this muscle-specific upregulation was blocked in the smyd1b?/?; hsf1?/? double-mutant embryos (S?X). Scale bar = 100 ?m.