FIGURE

Figure 2.

ID

ZDB-FIG-250130-46

Publication

Liu et al., 2023 - Tmem88 plays an essential role in pharyngeal pouch progenitor specification by inhibiting Wnt/β-catenin signaling

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Figure 2.

Excessive Wnt signal compromises PPP specification. (A) Expression of nkx2.3 in embryos treated with DMSO or BIO. The black dotted lines indicate the region where the PPPs are located. (B, C) Representative confocal sections showing Tg(nkx2.3:mCherry) embryos treated with DMSO or BIO (B). The PPPs are indicated by white dotted lines. Scale bar, 20 μm. Quantitative analysis of PPP cell numbers is shown in (C). The group values are expressed as mean ± SD. (D, E) Confocal images illustrating the specification of nkx2.3⁺ pouch progenitors from the pharyngeal endoderm. White arrowheads indicate the presence of GFP⁺/mCherry⁺ cells in Tg(nkx2.3:mCherry;sox17:GFP) embryos (D). Scale bar, 20 μm. Ratio of mCherry⁺ PPPs to the GFP⁺ pharyngeal endoderm of embryos treated with DMSO or BIO was achieved from three independent assays (E). The group values are expressed as mean ± SD.

Expression Data

Genes:	GFP mCherry nkx2.3
Fish:	TU ioz15Tg ioz15Tg; s870Tg
Condition:	chemical treatment by environment: 6-bromoindirubin-3'-oxime
Anatomical Terms:	pharyngeal endoderm pharyngeal pouch
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU ioz15Tg ioz15Tg; s870Tg
Condition:	chemical treatment by environment: 6-bromoindirubin-3'-oxime
Observed In:	pharyngeal endoderm pharyngeal pouch
Stage Range:	10-13 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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