Figure 2

CKAP4 is expressed in all glomerular cell types in human glomeruli.

Immunofluorescence staining of human kidney tissue for CKAP4 (green) and various markers for glomerular cells (red) to illustrate CKAP4 localization in the glomerulus. The first column shows CKAP4 staining. The second column shows, respectively: WT-1, marker for podocyte nuclei; synaptopodin, marker for podocyte foot processes; α-SMA, marker for mesangial cells; Ulex Europaeus Agglutinin I, marker for endothelial cell; agrin, marker for basement membrane. The third column shows colocalization; the fourth column contains zoomed area (white squares in the third column). Representative scale bars are placed below each column. All zoomed-in areas in the last column had an area of 65 μm². CKAP4, cytoskeleton associated protein 4; WT-1, Wilms tumor 1; α-SMA, smooth muscle actin.