Peroxisome biogenesis is compromised in adult pex1 zebrafish. (A) Confocal images of 5 μm liver sections from 7-month-old wild-type (WT) and pex1–/– fish stained for catalase (peroxisomal matrix marker) or PMP70 (peroxisomal membrane marker). WT hepatocytes display numerous punctate peroxisomes, whereas pex1–/– cells show a more diffuse catalase staining and fewer PMP70-positive puncta. Representative images were generated using Maximum Intensity Projection of the entire z-stack to enhance peroxisomal contrast. Secondary-antibody-only controls (Alexa488) verify staining specificity. Nuclei were counterstained with DAPI (cyan). Scale bars, 2 μm. (B) Quantification of peroxisome counts using Average Intensity Projection data. Bars represent means ± SD from three biological replicates (one section per fish; n = 3). Unpaired, two-tailed Student’s t-test: *p < 0.05; ns, not significant. (C) Heat-map of indicated fatty acid profiles measured by LC-MS in brain and liver of individual 7-month-old WT, pex1+/–, and pex1–/– fish. The numerical values shown on the right correspond to KO/WT log2 ratios and represent means of 3–4 biological replicates. Crosses on white background indicate missing values. Multiple unpaired Student’s t-test: *p < 0.033, **p < 0.0021, ***p < 0.0002, ****p < 0.0001.
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