FIGURE

Fig. 7

ID

ZDB-FIG-251230-34

Publication

Darche-Gabinaud et al., 2025 - Synaptic Gpr85 influences cerebellar granule cells electrical properties and light-induced behavior in zebrafish

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Fig. 7

Gpr85-KO impacts adult cerebellar GCs electrophysiological properties. A, Schematic representation of the experimental setup. Cerebellar horizontal sections (220 µm) were prepared, and GCs were identified based on GFP fluorescence. Recordings were made in cell-attached and whole-cell voltage–clamp configurations. The chart illustrates the percentage of GCs in cell-attached configuration that exhibited or not spontaneous activity. B, Quantification of GCs spontaneous activity in a cell-attached configuration. Firing patterns are shown, as well as the firing rates measured over a 60 s recording (gpr85GAL4/+, n = 13, vs gpr85GAL4/GAL4, n = 15; *p = 0.0347; unpaired t test with Welch's correction). C, Quantification of GCs passive properties (membrane capacitance, Cm; membrane resistance, Rm; membrane time constant, τ) in a whole-cell voltage–clamp configuration (gpr85GAL4/+, n = 26, vs gpr85GAL4/GAL4, n = 25; Cm, ns, p = 0.9132; τ, ns, p = 0.1724; M–W U tests; Rm, *p = 0.0113; unpaired t test with Welch's correction). Ce, cerebellum; GCL, granule cell layer. Data are presented as mean ± SEM (B, C).

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.