FIGURE

Figure S3.

ID

ZDB-FIG-260509-14

Publication

Gillotay et al., 2026 - The role of Nrf2 in thyroid maturation and hormone synthesis in vertebrate models

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Figure S3.

Loss-of-function of nrf2a does not affect Tg protein expression in zebrafish embryos.
(A, B, C, D, E, F, G, H, I, J, K, L) Immunofluorescence on 6 dpf Tg(tg:nlsEGFP) zebrafish embryos using antibodies targeting EGFP and TG (Cy3, red) reveals similar Tg protein levels in nrf2a homozygous mutant (n = 6) compared with their WT (n = 12) and heterozygous mutant siblings (n = 17) (A, B, C, D, E, F, G, H, I, J, K, L). (A, B, C) show whole head pictures of representative embryos of each genotype (ventral view, anterior to the right). (D, E, F, G, H, I, J, K, L) show an enlarged picture of the thyroid gland, (D, E, F) show GFP staining, (G, H, I) show Tg staining, and (J, K, L) show a merge of GFP and Tg staining. Scale bars: 50 μm. Ventral view, anterior to the left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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