FIGURE

Fig 4

ID

ZDB-FIG-260613-15

Publication

Lubash et al., 2026 - RNA Polymerase III subunit Polr3a is required for craniofacial cartilage and bone development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig 4

Cell death is increased within the craniofacial cartilage in polr3a^sa907/sa907 zebrafish.
Cell death (TUNEL, magenta) and proliferation (pH3, white) were quantified within pharyngeal arch 1 and 2 derived cartilage elements using sox10:egfp (green, outlined) to mark this domain. (A,B) At 3 dpf, there is a significant increase in cell death (C; *p = 0.016), but no change in proliferation (D; ns, p = 0.51). n = 12 controls‌‌, 12 mutants. (E,F) At 4 dpf, differences in the morphology of the craniofacial cartilage are visible, including a reduced basihyal, while cell death (G; ns, p = 0.08) and pH3 remain unchanged (H; ns, p = 0.94). n = 12 controls, 12 mutants. (I,J) By 5 dpf, there is a noted difference in cartilage morphology and increased cell death (K; **p = 0.008; n = 17 controls, 17 mutants), while pH3 is unchanged (L; ns, p = 0.48; n = 9 controls, 10 mutants), suggesting that differences are due to changes in cell death. Scale bar = 100 µm.

Expression Data

Antibodies:	Ab14-h3 Ab19-gfp
Fish:	ir937Tg (AB) polr3a^sa907/sa907; ir937Tg (AB)
Anatomical Terms:	ceratobranchial 1 cartilage ceratobranchial 2 cartilage
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	polr3a^sa907/sa907; ir937Tg (AB)
Observed In:	ceratobranchial 1 cartilage apoptotic process ceratobranchial 1 cartilage cell population proliferation ceratobranchial 2 cartilage apoptotic process ceratobranchial 2 cartilage cell population proliferation
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.