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Prednisone immersion treatment inhibited EIU inflammation in zebrafish. (A) Live cells were separated out from the retina cell suspensions of Tg(Mpx:GFP) line. Left, Flow cytometry was used to analyze the GFP-positive neutrophils. Right, quantification of percentages of GFP-positive cells in different conditions (n = 4 per group; mean ± SD; ***P < 0.001; ****P < 0.0001; ns, not significant; one-way ANOVA). (B) Left, flat mounts of retina around the optic disc in Tg(Mpx:GFP) line. Scale bar, 20μm. Right, quantification of the relative GFP-positive area in retinal flat mounts in different conditions (mean ± SD; n = 3 retinas per group; ****P < 0.0001; one-way ANOVA). (C) Transcript levels of proinflammatory genes of retina in zebrafish treated with 0.1% DMSO (vehicle-treated group) or 50μm prednisone (mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; unpaired t-test).
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