Fig 1

Three strains of zebrafish fgfr4 knockout mutants generated using CRISPR/Cas9.

(A) Schematic of generation of fgfr4 knockout zebrafish. Single cell wildtype zebrafish embryos were injected with fgfr4 guide RNA and Cas9 protein to generate mutant founders. Potential founders were outcrossed to isolate CRISPR/Cas9-mediated mutations, and putative crispant F1s were subjected to high resolution melt analysis to identify mutation sequences. (B) High resolution melt analysis (HRMA) of potential F1 mutants revealed three distinct clusters of potential fgfr4 crispants. (C) Schematic of wildtype Fgfr4 and predicted knockout strain protein lengths. Premature stop codons produced truncated Fgfr4 proteins with predicted length of 223, 228, and 215 amino acids respectively. Solid blocks indicate regions of wildtype Fgfr4 amino acid sequence alignment. The protein sequence aligned to the wildtype Fgfr4 protein ends at the same amino acid for all three alleles, with various additions of amino acids that do not align to the wildtype protein afterwards. Hatching indicates these additional amino acids.