Fig 3

Homozygous fgfr4 knockout (KO) zebrafish have no embryonic phenotype but are significantly smaller than wildtype zebrafish at three months post-fertilization (mpf).

(A) Representative images from a phenotypic analysis of embryonic zebrafish. Homozygous fgfr4 mutant zebrafish do not display an early embryonic phenotype. Each point represents an individual fish standard length, and the bar represents the mean. An unpaired two-tailed t-test with Welch’s correction was used to calculate the p value. Scale bar is 560.1 μm in 24 hpf images and 700.4 μm in 48 hpf images. Standard length quantification was performed with n = 15 embryos per group. (B) Phenotypic analysis of adult zebrafish. Homozygous knockout fgfr4 zebrafish are significantly smaller than wildtype zebrafish at three months post fertilization. Standard length quantification was performed with n = 27 WT fish and n = 34 fgfr4 KO adult fish. Each point represents an individual fish standard length, and the bar represents the mean. An unpaired two-tailed t-test with Welch’s correction was used to calculate p value. Scale bar is 1 cm. (C) Representative hematoxylin and eosin (H&E) staining of a sagittal section from female and male adult fgfr4 knockout zebrafish. Internal anatomy in mutant zebrafish is normal in both male and female adult fgfr4 knockout zebrafish. This was assessed in 5 female and 3 male fgfr4 knockout fish total. Scale bar is 1mm.