Identification of differentially expressed genes in smyd1b-deficient embryos by RNA-seq. (A) RNA-seq analysis was performed on smyd1b knockdown (KD) and knockout (KO) mutant embryos and their respective controls at 24hpf. Compared with embryos injected with the control-MO, knockdown of smyd1b resulted in upregulation of 469 gene expression and downregulation of 157 gene expression. Similarly, compared with WT/Heterozygous mutant sibling, 396 genes were upregulated and 75 were downregulated in the smyd1b KO mutant embryos. Among the up- or downregulated genes in smyd1b-KD and KO groups, 15 commonly upregulated and 10 commonly downregulated genes were identified by Venn diagram in smyd1b-KD and smyd1b-KO groups. (B) Heat map profiles show the fold changes of gene expression in the commonly upregulated (red) or downregulated (blue) genes. The top upregulated genes in smyd1b-KD and KO embryos encode mostly chaperones and co-chaperones. CTL1: Control-KD. CTL2:WT/heterozygotes. (C) The fold changes of expression among the upregulated hsp40, hsp70s, hsp90s chaperone and unc45b co-chaperone genes in smyd1b-KD and smyd1b-KO embryos compared with the respective control. adjp: Significant. Adjusted p values.** indicates significant difference at the adjusted p-values (adjp).
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