Figure 5

Assessment of Chtf18 mut HSPC by competitive transplantation.(A, B) The ability of adult Chtf18 mut bone marrow HSPC to repopulate hematopoiesis was assessed by competitive transplantation using allotype marked (CD45.1) competitor. 100,000 Lineage^– HSPC from CD45.2 WT or Chtf18 mut mice were mixed with an equal quantity of CD45.1 competitor cells and transferred into irradiated recipients. After 6 weeks, the hematopoietic reconstitution was assessed on explanted thymus (A) and spleen (B) cell suspensions by flow cytometry using anti-CD45.1 and anti-CD45.2 antibodies. Cell frequencies were displayed graphically as scatter plots. Statistical significance was assessed by multiple unpaired t tests. (C) The capacity of CD45.2 WT and Chtf18 mut HSPC to home to the bone marrow was assessed as above expect that bone marrow seeding was measured by flow cytometry 4 days after transfer. The number of transferred cells found in the recipient bone marrow was exhibited by scatter plot. Statistical significance was assessed by multiple unpaired t tests.