Clmp knockdown decreased smooth muscle-related gene expression in Zebrafish, and clmp mRNA partly rescued the knockdown phenotype in zebrafish embryos. (A) qRT-PCR analysis of the mRNA levels of clmp, fabp2, smoc2, myh11a, tagln, and acta2 expression in zebrafish embryos of control, control-MO, and clmp-MO groups at 72 hpf (each group, n = 3–6). The expression of gapdh gene was used as an internal control. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (B) The mortality rate of embryos with clmp-MO injection, clmp-MO, and clmp mRNA con-injection during different embryonic stages. (C) Gross morphology of zebrafish embryos at 72 hpf in three groups. Most embryos con-injected with clmp-MO and clmp mRNA were morphologically normal, whereas those injected showed growth defects. acta2, actin alpha 2; clmp-MO, clmp morpholino group, clmp-MO rescue group, co-injection of clmp morpholino and clmp mRNA group; control group, untreated group; control-MO, control morpholino group; Fabp2, fatty acid binding protein 2; myh11, myosin, heavy chain 11a; smoc2, SPARC-related modular calcium binding 2; tagln, transgelin.
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