FIGURE

FIGURE 2

ID

ZDB-FIG-250609-48

Publication

Mesika et al., 2025 - Impaired Proteostasis is Linked to Neurological Pathology in a Zebrafish NGLY1 Deficiency Model

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FIGURE 2

Adult ngly1^(−/−)fish feature cellular effects resulting from NGLY1 deficiency. (A) RT‐qPCR quantification of aqp1a.1 mRNA levels in zebrafish eye. ** T‐test p < 0.01 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3). (B–E) Western blot analysis of aqp1a.1 protein from adult zebrafish eyes (B, C); *** T‐test p < 0.001 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3), and brain (D, E) ** T‐test p < 0.01 (ngly1^(+/+)n = 3, ngly1^(−/−)n = 3). β‐actin was used as a loading control. (F–I) Transmission electron microscopy images showing mitochondrial fragmentation in the mutant zebrafish brain (white arrows). (J) Fragmented mitochondria were quantified by Form Factor metric, calculated by the formula: Form Factor = (4 × π × mitochondrial area)/(mitochondrial perimeter) [2]. ** T‐test p < 0.01, (N = 3 in each group).

Expression Data

Gene:	aqp1a.1
Antibody:	Ab1-aqp1a.1
Fish:	AB ngly1^{zf3678/zf3678} (AB)
Anatomical Terms:	brain eye
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ngly1^{zf3678/zf3678} (AB)
Observed In:	brain brain mitochondrion eye
Stage:	Adult

Phenotype Detail

Acknowledgments

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