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(a) Phylogenetic tree of LOXHD1 in zebrafish, medaka, pufferfish, stickleback, Xenopus, chicken, mouse, and human. The number at each node indicates the bootstrap probability (%). Scale bar, 0.1 substitutions per site. (b) Temporal analysis of LOXHD1a and LOXHD1b expression. Expression levels of LOXHD1a and LOXHD1b at 2, 3, and 5 dpf were measured by real-time qPCR analysis. The error bars show the standard deviation. (c, d) Spatial analysis of LOXHD1a and LOXHD1b expression in WT zebrafish by in situ hybridization of sense and anti-sense probes. Scale bars are 100 μm. (c) The ear vesicle was labelled with the LOXHD1a antisense RNA probe (black arrowhead). (d) The lateral line primordia were labelled by the LOXHD1b antisense RNA probe (red arrowhead)
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