Figure 7-supplement 1.

Biological assay of NACET activity on dnaaf1^-/- embryos.

(A) Theta angle (θ) measurement between the eye, the caudal end of the yolk extension and the tail extremity, corresponding to the embryo curvature index. (B) Lateral views of 60 hpf representative embryos showing axial curvature phenotypes. In untreated conditions, dnaaf1^-/- embryos show a characteristic curly tail-down (CTD) phenotype, whereas dnaaf1^+/+ siblings have a straight axis. Treatment at 27 hpf with 3 mM NACET suppresses the CTD phenotype of dnaaf1^-/- embryos and does not affect axis straightness of dnaaf1^+/+ siblings. Embryos were measured around 2.5–3 mm. (C–F) Rose plots representing the (360 – θ) angle of dnaaf1^+/+ and dnaaf1^-/- in untreated and 3 mM NACET-treated conditions. Embryos were treated from 27hpf to 2.5 dpf and θ angles were measured at the end of the treatment. Untreated dnaaf1^+/+ are represented in (C – dark green), and treated ones are in (D – light green). Untreated dnaaf1^-/- are in (E – blue) and treated ones are in (F – red). NACET treatment is able to rescue curly tail down phenotype in dnaaf1^-/- embryos without affecting the controls.