Figure 6

Rate of closure in siTMEM and siRNA cells. (A) Phase contrast (10×) images of transiently transfected BAOEC (siTMEM or control siRNA) cells undergoing migration 0 and 8 h post scratch. Seeded on 0.2% porcine gel; conducted in 0.2–0.5% Hi-FBS MEM media, 37 °C, and 5% CO₂. The gray line indicates the leading edge of migration. Scale 100 μm. (B) Rate of closure per well (siTMEM n = 11, control siRNA n = 10) was calculated and normalized to average rate of closure for siRNA per replicate (N = 3). A Mann–Whitney U test was performed, * p < 0.05. (U = 21, p = 0.016). (C) Confocal microscopy of the leading edge at 0 and 8 h post scratch (Gray: Phalloidin) (scale 20 μm). White arrows indicate lamellipodia during cell migrating at the leading edge at T8. (D) Percentage of migratory front cells containing lamellipodia (KD n = 125, control siRNA n = 132 cells). A chi-squared test was performed ** p < 0.001.