FIGURE

Fig. 3

ID

ZDB-FIG-251017-7

Publication

Xian et al., 2025 - ATP5F1A deficiency causes developmental delay and motor dysfunction in humans and zebrafish

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Fig. 3

Effect of p. Gly418Arg mutation on ATP5F1A mRNA and protein expression and subcellular localization. A. Relative expression level of ATP5F1A mRNA after HEK 293T cells were transfected with empty vector (NC), wild-type (WT) or mutant-type (MT, p. Gly418Arg) vector (qPCR detection, internal reference gene: GAPDH; data mean ± SD, n=3; **P<0.01, ****P<0.0001, one-way ANOVA). B. Immunoblotting analysis of ATP5F1A-FLAG protein (anti-FLAG antibody, internal reference: GAPDH). Representative images show the protein expression levels after empty vector, WT and MT transfection. C. Quantitative analysis of ATP5F1A-FLAG protein expression (ImageJ normalized to GAPDH; mean ± SD, n=3; **P<0.01, one-way ANOVA). D. Subcellular localization of ATP5F1A-FLAG in HEK 293T cells. Wild-type (WT) and mutant-type (MT) proteins (green, anti-FLAG antibody), mitochondrial inner membrane marker IMMT (red, anti-IMMT antibody) and cell nuclei (blue, DAPI staining). Scale bar, 10μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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