FIGURE

Fig. 4

ID

ZDB-FIG-251017-8

Publication

Xian et al., 2025 - ATP5F1A deficiency causes developmental delay and motor dysfunction in humans and zebrafish

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Fig. 4

atp5fa1 knockdown causes growth and development disorders in zebrafish. A Temporal expression profile of atp5fa1 mRNA during zebrafish embryonic development (qPCR detection, internal reference gene: β-actin). B The atp5fa1 mRNA expression levels in the control (control-MO) and atp5fa1-KD (atp5fa1-MO) larvae at 24hpf, 72hpf and 120hpf (mean ± SD, n = 15 embryos/group; ****P < 0.0001, Student t-test). C Immunoblotting analysis of Atp5fa1 protein (anti-ATP5F1A antibody, internal reference: Gapdh). Representative results show the difference in protein expression between the control group and the knockdown group. D Quantitative analysis of Atp5fa1 protein expression (ImageJ software normalized to Gapdh; mean ± SD, n = 3 independent experiments; ***P < 0.001, Student t-test). E Survival curves of control group and atp5fa1-KD group larvae (0-144hpf; total sample size n = 300 embryos/group). F Phenotypic comparison of 72hpf and 120hpf larvae. Scale bar, 300 μm. G Quantification of body length of 72hpf and 120hpf larvae (mean ± SD, n = 10; ****P < 0.0001, Student t-test)

Expression Data

Gene:	atp5f1a
Antibody:	Ab2-atp5a
Fish:	AB AB + MO2-atp5f1a
Knockdown Reagent:	MO2-atp5f1a
Anatomical Term:	whole organism
Stage Range:	1-cell to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-atp5f1a
Knockdown Reagent:	MO2-atp5f1a
Observed In:	pericardium trunk whole organism
Stage Range:	5-9 somites to Day 6

Phenotype Detail

Acknowledgments

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