FIGURE

FIGURE 2

ID

ZDB-FIG-251216-20

Publication

Chen et al., 2025 - Ddx3xa mutations drive cardiac defects in a zebrafish model via dysregulation of wnt/β-catenin signaling

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FIGURE 2

Spatiotemporal expression pattern of ddx3xa by whole-mount in situ hybridization and qRT-PCR analysis. (A–J) Spatiotemporal expression of ddx3xa at various developmental stages visualized by whole-mount in situ hybridization. Scale bar: 250 μm (applies to A–J). (A) 0.2 hpf; (B) 12 hpf; (C) 24 hpf; (D) 36 hpf; (E) 48 hpf; (F) 72 hpf; (G) Dorsal view, 24 hpf; (H) Dorsal view, 36 hpf; (I) Dorsal view, 48 hpf; (J) Dorsal view, 72 hpf. (K) Quantitative real-time PCR (qRT-PCR) analysis of relative ddx3xa transcript levels at various developmental time points (0.2, 3, 5.3, 8, 12, 24, 36, 48, 72, 96, 196 hpf). Expression levels were normalized to the 0.2 hpf time point. (n = 3; **P < 0.01, ***P < 0.001).

Expression Data

Gene:	ddx3xa
Fish:	AB
Anatomical Terms:	brain heart pectoral fin pectoral fin bud post-vent region skeletal muscle whole organism
Stage Range:	1-cell to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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