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Wnt signaling disruption in ddx3xa−/− zebrafish. (A) Expression of key Wnt signaling components in ddx3xa−/− larvae at 72 hpf by qRT-PCR (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT). (B) Whole-mount in situ hybridization (WISH) with tbx5 probe at 48 hpf. Numbers indicate identically stained embryos/total processed. Scale bar: 250 μm (applies to B–C). (a) WT dorsal view; (b) ddx3xa−/− dorsal view; (C) WT lateral view; (D)ddx3xa−/− lateral view. (C) WISH with bmp4 probe at 48 hpf. (a) WT ventral view; (b) ddx3xa−/− ventral view. (D)bmp4 expression levels in ddx3xa−/− embryos at 24, 48, and 96 hpf by qRT-PCR (n = 3; ***p < 0.001 vs. WT; ns: nonsignificant). (E)tbx5 expression levels in ddx3xa−/− embryos at 24, 48, and 96 hpf by qRT-PCR (n = 3; *p < 0.05, ***p < 0.001 vs. WT).
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