Figure 2

Conserved intra-/intergenomic synteny analysis supports the origin of the Kazald gene family in the 2R-WGD event ancestral to jawed vertebrates

(A) Illustration of the syntenic blocks containing the Kazald genes in four representative species. Various paralogous genes are connected via gray lines. Genes lost in a species are drawn with a dotted outline and no internal color. Kazald genes are bolded. Fgf genes were used as the syntenic block anchor when a Kazald gene was absent, and thus are also bolded.

(B) Consensus tree generated via BAli-Phy partitioned analysis using the amino acid sequences of the five listed gene families (see also Figure S3). Branch colors indicate the BI posterior probability at each node. Support values shown at nodes are BI posterior probabilities. Scale bar corresponds to the mean number of amino acid substitutions per site for each of the used gene families.

(C) Simplified diagram of individual Kazald gene maintenance across vertebrates, along with the relationships of the different lineages. Colors of Kazald genes and the syntenic blocks correspond to each other. Dual boxes for Kazald2 in Eloposteoglossocephala represent the presence of Kazald2a and Kazald2b in the contained superorder Elopomorpha. Urodela is listed with the four Kazald genes present, as every examined species with a sequenced genome was found to possess all four genes.