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Kazald2 is expressed during regeneration in species throughout the osteichthyan lineage (A) Quantification of Kazald1 and Kazald2 expression over the course of axolotl limb regeneration. (A′ and A″) Whole-mount in situ hybridization of Kazald2 in 5 dpa and intact axolotl limb. Scale bar, 500 μm. (B) Quantification of Kazald2 expression in the regenerating fins and tail of lungfish and bichir. (C) Quantification of Kazald2 expression in the regenerating mandible, spine, brain, and retina of axolotl, and the regenerating lens of newt. (D) RT-qPCR examining Kazald2 expression in the regenerating limbs of the lungless salamander. (E) Quantification of Kazald expression over the course of lamprey spine regeneration. (F) Quantification of Kazald expression over the course of amphioxus tail regeneration. (G) Quantification of kazald2 and kazald3 expression in the regenerating heart and caudal fin of zebrafish, and Kazald1 and Kazald4 expression in the regenerating tails of green anole and tokay gecko. (H) Whole-mount in situ hybridization of kazald2 in regenerating caudal fin of adult zebrafish at 2 dpa. Dashed line indicates the amputation planes. Scale bar, 250 μm. (I) Quantification of Kazald1 and Kazald2 expression over the course of axolotl embryo development, and in the developing limb bud of axolotl larvae. PRJ IDs indicate the publicly available RNA-Seq datasets that generated the raw data for the listed tissues. Dots represent biological replicates in examined datasets, and error bars represent standard deviation when calculable. CPM = counts per million, hpa = hours post-amputation, dpa = days post-amputation, wpa = weeks post-amputation, hpi = hours post-injury, dpi = days post-injury, Reg. = regenerating, Uninj. = uninjured, Dor. = dorsal, Ventr. = ventral. Data are represented as mean ± SD.
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